University of Sheffield, Sheffield, UK, 1991-1995, Ph.D., Potassium Channels
University of Vermont, Burlington, VT, 1995-1997, Postdoc., Gastrointestinal Physiology
University of Vermont, Burlington, VT, 1997-1999, Postdoc., Vascular Physiology
The focus of my laboratory is to study mechanisms that regulate the diameter of small arteries and arterioles that modulate systemic blood pressure and organ blood flow. Smooth muscle cells within the vascular wall determine arterial diameter. Our major focus is to investigate the functional significance of arterial smooth muscle cell plasma membrane and intracellular ion channels and local and global calcium signals in regulating arterial smooth muscle cell contractility. Research in the laboratory involves a multi-faceted approach, studying events at molecular, cellular and intact artery levels. Techniques include patch clamp electrophysiology, rapid confocal calcium imaging, conventional calcium imaging, immunofluoresence, FRET, diameter measurement of pressurized arteries, use of knockout models, and molecular biology. Please click on My Laboratory for more detailed information.
04/01/02 - 03/31/13, NIH R01 HL67061, Calcium Signaling in Cerebral Arteries. P.I. 06/01/09 - 05/31/13, NIH R01 HL94378, Calcium Channels in Arterial Smooth Muscle Cells. P.I. 07/01/11 - 06/30/16, NIH R01 HL110347, Arterial Smooth Muscle Chloride Channels, P.I. 04/01/85 - 03/31/12, NIH R01 HL34059, Carbon Monoxide in Newborn Circulation, Co-I. 07/01/09 - 06/01/14, NIH R01 AA11560, Ethanol Actions on Slo1 Channels from Arteries versus Brain. Co-I. 07/01/10 - 06/30/15, NIH R01 HL104631, Vasodilation via Selective Pharmacological Targeting of BK channel Beta1 Subunits. Co-I. 08/01/90 - 07/01/15, NIH R01 HL42851, Hydrogen Sulfide in Newborn Cerebral Circulation, Co-I.
1.Xi Q, Cheranov SY,Jaggar JH. Mitochondria-derived reactive
oxygen species dilate cerebral arteries by activating Ca2+
sparks.Circ Res 2005;
97:354-362.Editorial by D.D. Gutterman.
2.Jaggar JH, Li A, Parfenova H, Liu J.,
Umstot ES, Dopico AM, Leffler CW. Heme is a carbon monoxide receptor for
large-conductance Ca2+-activated K+ channels.Circ Res 2005; 97: 805-812.
X., Liu, J., Asuncion-Chin, M., Blaskova, E., Bannister, J.P., Dopico, A.M. and
Jaggar J.H.A novel CaV1.2
N-terminus expressed in smooth muscle cells of resistance-size arteries
modifies channel regulation by auxiliary subunits.J Biol Chem 2007; 282: 29211-29221.
4.Li, A., Xi, Q., Umstot, E.S, Bellner, L., Laniado-Schwartzman,
M., Jaggar, J.H., and Leffler, C.W.Astrocyte-derived
CO is a diffusible messenger that mediates glutamate-induced cerebral
arteriolar dilation by activating smooth muscle cell KCa
channels.Circ Res 2008; 102(2):234-41.
Q. Adebiyi, A., Zhao, G, Chapman, K.E., Waters, C.M., Hassid, A., and Jaggar,
J.H.IP3 constricts cerebral
arteries via IP3 receptor-mediated TRPC3 channel activation and
independently of sarcoplasmic reticulum Ca2+ release.Circ Res 2008;102(9): 1118-1126.
A., McNally, E.M., and Jaggar, J.H. Sulphonylurea receptor-dependent and -independent pathways mediate vasodilation induced by KATPchannel
openers.Mol Pharmacol 2008; 74:
J.P., Adebiyi, A., Zhao, G., Narayanan, N., Thomas, C.M., Feng, J.Y.,Jaggar, J.Y. Smooth muscle cell alpha2delta-1 subunits are essential for
vasoregulation by CaV1.2 channels. Circ Res 2009; 105: 948-955.
A., Zhao, G., Narayanan, D., Thomas, C.M., Bannister, J.P., Jaggar, J.H.Isoform-selective physical coupling of TRPC3
channels to IP3 receptors in smooth muscle cells regulates arterial
contractility. Circ Res2010; 106(10): 1603-1612.
9.Narayanan, D., Xi, Q, Pfeffer, L, Jaggar, J.H.Mitochondria control functional CaV1.2
expression in smooth muscle cells of cerebral arteries.Circ Res 2010; 107(5): 631-641. Circ Res Editors' Pick and Faculty of 1000 Must Read Article.
G, Neeb, Z, Leo, MD, Pachuau, J, Adebiyi, A., Ouyang, K., Chen, J., and Jaggar
J.H. Type 1 IP3 receptors activate BKCa channels via
local molecular coupling in arterial smooth muscle cells.J Gen Physiol 2010; 136(3): 283-291. JGP Journal Club
Article by Mujica and Gonzalez and JGP
A., Narayanan, D., and Jaggar, J.H.Caveolin-1 assembles type 1 inositol 1,4,5-trisphosphate receptors and
canonical transient receptor potential 3 channels into a functional signaling
complex in arterial smooth muscle cells.J. Biol. Chem. 2011; 286 (6): 4341-4348. Editors Choice:Coupled by Caveolin.Science
Signaling, Vol. 4, Issue 160, p. ec50. Featured in NAVBO
Publications Alert, March 2011.